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Job: Research Technician III - 106929, Duke Human Vaccine Institute, Durham, NC

General Information

Job title:
Research Technician III - 106929
Job location:
Durham, NC  27710 United States
Requisition code:
Date posted:
Job category:
Research and Development
Employment type:
Full time

Job Description


The Duke Human Vaccine Institute (DHVI) continues to lead cutting edge vaccine research against infectious diseases that impact global health. Basic science discoveries made using a variety of human and pathogen-derived proteins, designed, expressed and purified by a collaborative DHVI team have contributed to the identification of vaccine and therapeutic candidates currently being produced in Good Manufacturing Practice facilities for early phase clinical trials.

The proteins derived from HIV envelope will be designed and expressed as candidate vaccine immunogens that will also be used to probe B cells in humans and rhesus macaques in an effort to characterize B cells that can be stimulated by these immunogens. These proteins will also be used to functionally characterize B cell derived antibodies. Thus, our efforts are aimed at identifying and characterizing candidate immunogens and the target B cells to be harnessed by an effective HIV vaccine.


• Perform the following experimental activities:
o Neutralization assays of HIV-1 infection in tissue culture
o Compile data reports on neutralization titers
o Calculate neutralization titers using current Excel macros
o Input assay data into online data portal
o Propagate viruses for use in assays
o Virus titration assays
o Transfection of tissue culture cells
o Maintain tissue culture cells using aseptic technique
o Luminescence assays
o Safely handle infectious material from human or animal sources
• Understand and comply with all safety standards and procedures to ensure a safe working environment for all laboratory staff
• Design, execute, analyze, and archive experiments in a manner that is scientifically justifiable, reliable and understandable to others
• Help manage protein inventories
• Keep lab equipment, chemicals reagents, and supplies in excellent order
• Perform routine lab maintenance; cleaning, organizing, and restocking supplies
• Track work and compliantly order materials and reagents associated with numerous projects under multiple grants
• Learn new techniques from colleagues, develop new techniques for the lab, and teach them to others
• Review primary data with your supervisor
• Collaborate with other staff and perform other duties as requested by the Principal Investigator or senior lab staff
• Closely follow all standard operating procedures

Job Requirements

• Education/Training: Work generally requires skills acquired through the completion of a bachelor's degree in botany, biology, zoology, psychology or other science related scientific field program.
• Experience: Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
(Job number: 4037747)
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