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Job: Research Associate - Flow Cytometry, Precision BioSciences, Durham, NC
Research Associate - Flow Cytometry
Durham, NC 27701 United States
302 E Pettigrew St
Research and Development
Precision BioSciences is seeking a Research Associate whose primary focus will be running flow cytometry within the Cell Therapy Development Team. The Research Associate will provide bioanalytical support for Clinical Sample Analysis and Cell Therapy Process Development projects and will contribute to bioanalytical development efforts for CAR T-cell projects by providing sample preparation and analysis in a timely manner.
Essential Duties and Responsibilities:
Isolate and characterize leukocytes from human peripheral blood
Prepare isolated leukocytes for analysis by flow cytometry
Prepare nucleic acid samples from isolated leukocytes
Cytokine analysis of human plasma samples
Perform aseptic mammalian primary or secondary cell culture, cryopreserve and manage cell inventory
Multicolor flow cytometry assay execution and analysis
Cell based assay execution and analysis
Execute established assays and analyze generated data according to established SOPs and report results of analysis to Cell Therapy Team Leaders on a timely manner.
Coordinate with Cell Therapy Team Leaders to plan experiments that need to be carried out to achieve Precision’s goals for developing T Cell Therapies
Coordinate with team members to plan and carry out experiments to achieve Precision’s goals for developing T Cell Therapies
Work collaboratively on aspects of experiments to be performed by other members of the team as part of a larger ongoing experiment
Assist in general operation of the Cell Culture facility
Involves some work during off-peak hours when project demands dictate. This will include occasional weekends, holidays, and/or evenings.
Coordinate with group leaders to ensure the following:
-SOPs are written and updated for techniques and assays
-Equipment is properly maintained
-Consumables are ordered and stocked as needed
-Cells and other reagents are available for experiments as needed
BS in cell & molecular biology, immunology or related field with 2+ years of related biopharmaceutical industry experience and/or training, or MS in cell & molecular biology, immunology or related field; or equivalent education and training
Familiarity with T cell biology
Extensive experience with antibody-based assays (Flow cytometry and ELISA)
Experience in handling and analysis of cells, antibodies, and nucleic acids
Demonstrated experience with multiparameter flow cytometric analysis
Excellent collaboration and communication skills
Able to work some evenings/weekends as needed
Hands-on experience with immune cell activation and characterization assays such as multi- parameter flow cytometry, ELISA, ELISpot, Luminex, or MSD
Experience with primary T cell culture, expansion and characterization
Proficiency with FlowJo, Prism, and Microsoft office applications
Minimal travel is required for this position. Opportunities to participate in conferences, seminars and workshops will be available and encouraged.
This is a laboratory-based position located at the Precision BioSciences, Inc. headquarters in Durham, NC
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