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Job: Vector Production Associate, StrideBio, Research Triangle Park, NC

General Information

Job title:
Vector Production Associate
Job location:
Research Triangle Park, NC  27709 United States
Requisition code:
Date posted:
medical, dental, vision, 401k with employer match, paid time off, company paid holidays
Job category:
Manufacturing and Production
Employment type:
Full time

Job Description

StrideBio is a product-driven, fully integrated gene therapy company focused on creating and developing innovative genetic medicines with life-changing or curative potential for patients with devastating conditions, including monogenic rare disease and beyond.

StrideBio leverages its proprietary structure-inspired adeno-associated virus (AAV) vector engineering platform (STRIVE) to create unique and differentiated vectors that improve upon naturally occurring AAV serotypes to overcome current limitations of first-generation gene therapies. Combined with our gene construct expertise and in-house manufacturing capabilities, StrideBio is positioned to generate best-in-class genetic medicines that allow more patients to benefit with maximum efficiency.

StrideBio is leveraging its STRIVE platform to develop AAV vectors that improve upon naturally occurring AAV serotypes, overcoming current limitations of first-generation gene therapies. StrideBio's vectors have been engineered to present a unique sequence of modified surface domains that avoid recognition by pre-existing neutralizing antibodies, creating potential to expand the treatment-eligible population. These same surface residues which are involved in neutralizing antibody interactions also influence other functional aspects of the vectors. As a result, StrideBio's unique and differentiated vectors also allow for:

Improved tissue targeting and de-targeting
Increased potency and transduction efficiency
Improved manufacturability at scale
StrideBio's product-driven approach includes fully integrated discovery, development and manufacturing capabilities. In addition to developing novel vectors, our gene construct expertise enables the development of multiple genetic medicine modalities to advance lead product candidates, with the in-house manufacturing capability to scale and support clinical development.


The purpose of this position is to perform experimental tasks as part of a team with high reproducibility and attention to detail. The production team provides a critical service for other groups (preclinical and vector engineering) within StrideBio, allowing the company to test new vectors candidates.

This position is responsible for production of recombinant adeno-associated virus (rAAV) vectors via maintenance of mammalian and insect cell lines, transfection, infection, and downstream purification of rAAV by chromatography and ultracentrifugation. You will utilize shake flasks, WAVE bioreactors, and stir-tank bioreactors for scale up experiments. You will also be directly involved in scalable downstream purification technologies for rAAV using FPLC systems, tangential flow filtration, and centrifugation. Work activities will also include compiling experimental data and contributing to protocols and study reports with supervision. It is expected that you will work as a member of the production team while accomplishing the timely completion of assignments and reporting of unexpected technical issues.


Maintain cell growth, transfection, and infection of mammalian and insect cells for baculovirus and rAAV manufacturing using shake flasks and single-use bioreactors
Execute downstream purification technologies related to rAAV purification using chromatography (affinity and ion-exchange) and filtration processes including microfiltration, depth filtration, tangential flow filtration, and sterile filtration
Assist in the production of preclinical and clinical materials in a laboratory or cleanroom settings
Perform vector characterization assays (qPCR, SDS-PAGE, Western blotting, etc.) on in-process and final samples on as needed basis
Compile experimental data with minimal oversight and contribute results for publications or reports
Interface with other departments (Quality Control, Process Development, Programs, etc.) as necessary

Job Requirements


B.S. in Biochemistry, Chemistry, Biology or related discipline
4+ years' experience required. Prefer that experience is in a biotech or pharma setting related to biological manufacturing
Technical proficiency required for aspects of cell line growth and production of biological product
Working knowledge of upstream production techniques; experience with WAVE bioreactors or stir-tank bioreactors is preferred
Working knowledge of downstream purification techniques; experience with GE AKTA systems (Pure, Avant, Pilot) FPLC is preferred
Experience with production of biologics and performing bioassays is preferred
Experience working in a cGMP environment is preferred
Must have strong attention to detail with ability to effectively plan
Strong written, verbal and organizational skills are critical
Must be a self-motivated and driven professional who can effectively perform in a rapidly changing, small company environment
Must be able to work independently, but is also a team player with strong collaborative skills


We offer a competitive salary and excellent benefits including stock options, PTO, Medical, Dental, Vision, 401k match, disability and life insurance. Additionally, we provide the following perks:

Complimentary onsite gym membership
Fully stocked kitchen
Socials, happy hours and other events throughout the year
Access to an onsite full-service restaurant (Mid-2020)
Access to an onsite massage therapist (Mid-2020)

For more information, or to apply now, you must go to the website below. Please DO NOT email your resume to us as we only accept applications through our website.
(Job number: 3881873)
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