Job: Scientist - Cell Therapy Discovery, Precision BioSciences, Durham, NC
Scientist - Cell Therapy Discovery
Durham, NC 27701 United States
Paid Medical, Dental, Vision
8 weeks of Paid Time Off per Year
Research and Development
The Scientist works within the Cell Therapy Discovery Team to advance Precision’s approach to engineering T cells and producing cell therapy products with potential applications in immuno-oncology and additional therapeutic areas. The Scientist carries out research projects that support development of T cell therapy platforms in accordance with Precision’s goals. The Scientist position is lab-based and will share facilities and resources with the Cell Therapy Discovery and CMC teams.
Essential Duties and Responsibilities
Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions of this position. This list contains the major duties and requirements of the job and is not all-inclusive. Incumbent(s) may be expected to perform role-related duties other than those contained in this document.
Provide leadership in advancing chimeric antigen receptor (CAR) and T cell receptor (TCR) internal research and development programs and external collaborations from construct design and cell production through functional analyses
Coordinate with Cell Therapy Associate Director and senior team members to plan and execute experiments to achieve Precision’s goals for developing T Cell Therapies
Generation and characterization of CAR T and/or recombinant TCR T cells
Plan and perform cell based assays to evaluate new CAR and TCR constructs, and other novel gene-edits in engineered T cells, including in vitro analyses of proliferation, cytotoxic activity, and cytokine release
Design constructs with novel architecture to impart addition desirable properties to CAR and TCR cell therapy products, such as for expression of additional proteins or molecules anticipated to enhance the function of next-generation engineered T cells, dual-specificity, safety switches, etc.
Coordinate in vivo experiments in appropriate murine models to evaluate efficacy and safety of gene-edited T cells
Coordinate with other scientists, research assistants and research associates to plan and execute collaborative experiments supporting Cell Therapy research projects
Contribute to driving the scientific direction of the Cell Therapy group by attending conferences, studying related literature, and incorporating new concepts into experimental design
Report results to Cell Therapy team, upper management, external parties
Share new data, file reports as needed, maintain updated electronic lab notebook records
Assist in publishing, patenting or presenting data as needed
Assist in general operation of the Cell Culture facility
Coordinate with Cell Therapy and CMC teams to make sure:
SOPs are written and updated for techniques and assays
Equipment is properly maintained
CO2 and LN2 tanks are filled when needed
Shared resources are utilized appropriately between teams
Consumables are ordered and stocked as needed
Cells and other reagents are available for experiments as needed
The requirements listed below are representative of the knowledge, skill, and/or ability required to perform this job successfully.
PhD in cellular & molecular biology/immunology with a minimum of 4 years related academic or industry experience and/or training.
MS in cellular & molecular biology/immunology with a minimum of 7 years related academic or industry experience and/or training.
BS in cellular & molecular biology/immunology with 10 years related academic or industry experience
Familiarity and experience with siRNA, shRNA, protein engineering, and gene editing a plus
Ability to read, analyze, and interpret common scientific journals. Ability to define problems, collect and analyze data, establish facts, and draw valid conclusions. Should have knowledge of Microsoft Office software suite.
Ability to respond to common inquiries or complaints from customers, regulatory agencies, or members of the business community.
Ability to apply advanced mathematical concepts such as exponents, logarithms, quadratic equations, and permutations.
Ability to apply mathematical operations to such tasks as frequency distribution, determination of test reliability and validity, analysis.
Demonstrated expertise in primary T cell culture is required, with preference given to candidates with in-depth knowledge and experience with cell-based assays of lymphocyte function, including proliferation, cytotoxicity, and cytokine release assays
Experience with multi-parameter flow cytometric analysis is required. Accompanying data analysis software proficiency is required
Strong preference given to candidates with extensive knowledge of TCR and immunoglobulin rearrangement and sequence analysis and previous experience designing constructs for expression of recombinant TCRs and CARs
Experience and knowledge of molecular biology techniques is desired, including: PCR, restriction digestion & cloning, primer design, plasmid isolation and analysis, bacterial transformation and screening, extraction of genomic DNA, DNA sequencing and analysis, and proficiency with accompanying data analysis software
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