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Job: Cell Line Engineering Scientist, Asklepios BioPharmaceuticals Inc, Durham, NC

General Information

Job title:
Cell Line Engineering Scientist
Job location:
Durham, NC  27709 United States
Requisition code:
Date posted:
Competitive Salary and Bonus Opportunity
Job category:
Research and Development
Employment type:
Full time

Job Description

About us:

When AskBio’s scientific founder, Dr. Jude Samulski, discovered how the Adeno-Associated Virus (AAV) could be safely used to deliver corrected genes to cells with genetic defects, it propelled one of the most exciting and inspiring fields in medical research today. Now the foundation for an entire industry, it also inspired the development of a world-leading gene therapy platform that includes the Pro10TM cell line, an extensive capsid library, and new methods for lowering the cost of delivery.

We know gene therapy is a highly complex business. But seeing the impact that gene therapy can have on patients suffering from incurable diseases fuels a never-ending passion to continually advance AAV gene therapy technology. We embrace the work that companies like Pfizer/Bamboo, Novartis/AveXis, and Roche/Spark are doing to advance gene therapy development as we all share a common goal: erase genetic disease.

We are a company on the forefront of the industry, guided by the vision and unparalleled expertise of Dr. Samulski, the founder of AAV gene therapy. We invite all researchers, advocacy groups, patients and their families to join us on this mission to advance promising gene therapies and to change the face of health care.

We are excited for our next Cell Line Engineering Scientist to join the AskBio team!

About the role:

The Cell Line Engineering Scientist will be to investigate cellular and molecular mechanisms for optimal recombinant Adeno-associated virus vector production. This position offers the opportunity to impact a growing portfolio of gene therapy programs in a fast-paced and innovative research environment.

Responsibilities and Accountabilities

• Lead, design and execute cell line development, clone screening, selection and stability determination for rAAV producer/stable cell lines.

• Design and develop novel ways of integrating and controlling gene expression of viral genes in stable cell lines.

• Support early stage discovery efforts for lead candidate identification.

• Implement high throughput and cutting-edge cell line development technologies to optimize platforms and shorten cycle time.

• Train and manage team members and delegating tasks appropriately.

• Provide input on the acquisition of capital equipment.

• Assist in the generation of project and cost proposals, timelines, and work plans by applying scientific knowledge and project management experience.

• Maintain a current awareness of scientific literature and actively applies new concepts and technologies as appropriate.

Job Requirements

About you:

MS or Ph.D. in Biological Sciences specializing in Cell Biology, Molecular Biology, Biochemistry, Virology, Chemical engineering or Biomedical Engineering with a minimum of 5-7 years’ experience in an industrial/research laboratory developing mammalian cell lines.

MS or PhD in Bioanalytical Chemistry, Biology, Biochemistry, Virology or related field with at least 1-3 years’ experience.

Excellent aseptic skills, experience with vector engineering, high-throughput gene expression technology and cell line engineering is a must.

Experience in developing novel molecular and analytical tools for use during cell line development is a must.

Proficient in a broad range of cell and molecular biology techniques including cloning (eg. Gibson and Golden Gate Assembly) and gene editing technologies such as CRISPR/Cas9.

In-depth knowledge and extensive hands-on experience in mammalian cell line engineering using gene editing/KD approaches (CRISPR, Meganuclease and si/shRNA). Experience with CRISPR knock-in is strongly preferred.

Experience with cell characterization technologies including GRO_Seq, DNA_Seq, qPCR, Western blot, ELISA, etc.

Hands on experience with FAC-Sorting and Flow cytometry assay development. Ability to multitask among multiple projects and teams and work independently in a fast-paced, highly interactive environment.

Excellent written and verbal communication, work planning, data analysis and record keeping skills (with strong attention to detail) will be required in addition excellent interpersonal skill.

More About you:

Hands on knowledge of DOE, JMP, FlowJo and GraphPad statistical software for use in design of technology development studies in a highly desirable.

Experience in regulatory IND filing and working knowledge of US/EU CLD requirements is highly desirable.

Must have a passport and are able to physically travel to remote locations, including, but not limited to, international and remote locations.

Must be able to tolerate normal office climatic conditions associated with daily activities.


We offer a competitive total rewards package. Are you our next great discovery?
(Job number: 3840503)
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